Tau-441 (2N4R) Wild-Type Pre-formed Fibrils (Baculovirus/Sf9)

Brain-specific tau isoforms vary in the number of N-terminal inserts and C- terminal repeat domains due to alternative splicing of exons; the 2N4R isoform of tau is expressed in adult brain yet is absent from the fetal brain (1). Tau (tubulin-associated unit) is normally located in the axons of neurons where it stabilizes microtubules. Tauopathies such as Alzheimer’s Disease (AD) are characterized by neurofibrillary tangles containing hyper-phosphorylated tau fibrils (2). Hyper-phosphorylated tau can be generated via expression in the Sf9/Baculovirus system, with up to 20 sites confirmed by mass spectrometry and Western Blots with phospho-specific antibodies(3). Sf9/Baculovirus-expressed Tau 2N4R Pre-Formed Fibrils are generated without heparin.