In 2020, Navinci introduced UnFold, the next generation of its proprietary in situ PLA technology, through the NaveniFlex product line. This breakthrough enables researchers worldwide to achieve in situ detection of protein interactions and post-translational modifications that are beyond the capabilities of standard techniques like Immunofluorescence (IF) and Immunohistochemistry (IHC).

At the forefront of innovation in spatial proteomics, Navinci’s Naveni® in situ PLA technology offers unmatched sensitivity and specificity in detecting and quantifying protein interactions and post-translational modifications. This powerful tool enables researchers to study proteins in their native environment, providing deeper insights into their dynamic interplay.

By delivering reliable and innovative tools for visualizing and analyzing protein interactions, Navinci advances the understanding of cell biology and brings precision to the rapidly evolving field of spatial proteomics.

Products illuminating function in Spatial Proteomics

Naveni® in situ proximity ligation technology redefines fluorescence-based in situ methodologies to visualize and quantify proteins, their interactions, and modifications directly at the molecular level. This technology enables detection of even low-abundance proteins without the need for overexpression or alteration of the cell's natural environment. By preserving the integrity of the biological context, Naveni® technology generates data that deepens the understanding of biological mechanisms in both healthy and diseased tissues, responses to therapeutic interventions, and changes within the cellular microenvironment.

Naveni® technology delivers clean, high-resolution images that are ready for precise quantification, ensuring maximum insights from every analysis.

Key Benefits of Naveni® Technology

• Clear Target Detection: Achieve high specificity and accuracy.
• Consistency in Staining: Reliable results across experiments.
• Reproducibility: Ensures consistent outcomes, critical for robust research.
• Detection of Low-Abundance Proteins: Study proteins without altering their natural context.
• Protein-Protein Interaction Detection: Unveil critical molecular interactions.
• Specific Post-Translational Modification Detection: Employ pan-specific antibodies for precise analysis.

Applications

Naveni® technology is designed for academic and industrial researchers engaged in:

• Immuno-Profiling: Understanding immune system dynamics.
• Signaling Profiling: Mapping cellular communication pathways.
• Drug Testing: Evaluating therapeutic compounds.
• Treatment Validation: Confirming efficacy of interventions.
• Biomarker Discovery: Identifying novel disease markers.
• Diagnostics: Enhancing precision in disease detection.

Workflow

With Naveni®, researchers can push the boundaries of spatial proteomics, gaining insights that drive innovation in cell biology and therapeutic development.

All Navinci’s kits are based on the novel, proprietary Naveni® in situ Proximity Ligation Technology, which enables precise detection of proteins, protein-protein interactions, or protein modifications in cultured cells fresh-frozen and FFPE samples. Features of the kits: Sensitive detection of protein interactions with high specificity and low background. Also kits available for the Leica BOND RX platform.

Explore the collection of video presentations of posters highlighting the benefits of the in situ proximity ligation assay. These presentations showcase various collaborative projects involving Navinci and offer detailed insights into recent advancements in the field.

The kits of Navinci can be used to detrect proteins, protein-protein interactions, or protein modifications in cell or tissue samples.

The target-specific kits includes carefully selected and validated Navenibodies with specific target protein detection for PD1/PD-L1 and CD8/MHC-I. No extra antibodies are needed. Also available for the Leica BOND RX platform.