IBA Lifescience

Sanbio, authorized distributor of the Benelux!

IBA Lifesciences GmbH, located in Göttingen, Germany, is a biotechnology company providing products for life science research in academia and industry. Leveraging their exclusive Strep-tag® technology, they engineer cutting-edge tools for cell and protein isolation.

View all IBA Lifescience products

Product groups for IBA Lifescience

“High performance research tools for cell and protein isolation,,

Product portfolio

Affinity Chromatography
Antibody Development
Antigen-specific Cell Isolation
Biotin Blocking
Cell Isolation
Cloning
ELISA
Flow Cytometry

High-Throughput Purification
Protein Purification
Protein-Protein Interaction
Pull-Down
SPR & BLI
Staining & Detection
Transfection
Western Blot

Strep-tag® technology

Cutting-edge tools for high-performance research in cell and protein isolation are enabled by the Strep-tag® technology, leveraging the robust interaction between biotin and streptavidin.

This technology harnesses the specificity and controllability of the interaction between the synthetic Strep-tag®II peptide and engineered streptavidins like Strep-Tactin®, renowned for its stability. By occupying the biotin binding site, Strep-tag®II forms a reversible interaction with engineered streptavidins, Strep-Tactin® and Strep-Tactin®XT easily disrupted by excess biotin.

The Strep-tag®II, comprising eight amino acids (Trp-Ser-His-Pro-Gln-Phe-Glu-Lys), and its variant, Twin-Strep-tag® is composed of 28 amino acids inluding two of these motifs in tandem connected by a linker. Both exhibit varying affinities for Strep-Tactin® derivatives. Strep-tag®II shows significantly higher affinity for Strep-Tactin® compared to streptavidin, with an affinity nearly 100 times stronger. The development of Strep-Tactin®XT further enhances affinity, which shares nanomolar affinity with Strep-tag®II and picomolar affinity for Twin-Strep-tag®.

Due to variations in binding strength among different tag-ligand combinations, the Strep-tag® system has emerged as a versatile tool for isolating proteins and cells.

The Strep-tag® fused to recombinant proteins facilitates efficient one-step purification when immobilized on Strep-Tactin®.
When attached to antibody-derived Fab fragments or nanobodies, the Twin-Strep-tag® interacts with multimerized Strep-Tactin®, enabling the capture and release of target cells based on their surface markers or antigen specificity.
Strep-Tactin® (or Strep-Tactin®XT) can be conjugated to microplates, fluorophores, or chips, wich makes this tool suitable for a wide range of analytical applications following the isolation of target material, such as detection, immobilization, and interaction studies.