Sanbio, authorized distributor of the Benelux!
IBA Lifesciences GmbH, located in Göttingen, Germany, is a biotechnology company providing products for life science research in academia and industry. Leveraging their exclusive Strep-tag® technology, they engineer cutting-edge tools for cell and protein isolation.
“High performance research tools for cell and protein isolation,,
Cutting-edge tools for high-performance research in cell and protein isolation are enabled by the Strep-tag® technology, leveraging the robust interaction between biotin and streptavidin.
This technology harnesses the specificity and controllability of the interaction between the synthetic Strep-tag®II peptide and engineered streptavidins like Strep-Tactin®, renowned for its stability. By occupying the biotin binding site, Strep-tag®II forms a reversible interaction with engineered streptavidins, Strep-Tactin® and Strep-Tactin®XT easily disrupted by excess biotin.
The Strep-tag®II, comprising eight amino acids (Trp-Ser-His-Pro-Gln-Phe-Glu-Lys), and its variant, Twin-Strep-tag® is composed of 28 amino acids inluding two of these motifs in tandem connected by a linker. Both exhibit varying affinities for Strep-Tactin® derivatives. Strep-tag®II shows significantly higher affinity for Strep-Tactin® compared to streptavidin, with an affinity nearly 100 times stronger. The development of Strep-Tactin®XT further enhances affinity, which shares nanomolar affinity with Strep-tag®II and picomolar affinity for Twin-Strep-tag®.
Due to variations in binding strength among different tag-ligand combinations, the Strep-tag® system has emerged as a versatile tool for isolating proteins and cells.
