The Quick-16S Plus NGS Library Prep Kit (V3-V4, UDI) is the fastest and simplest NGS library prep targeting the V3-V4 region of the 16S rRNA gene for high-throughput sequencing. The automation-friendly protocol utilizes a single qPCR/PCR for combined targeted amplification and barcode addition using specially designed primers. After pooling by equal volume, a single clean-up of the final library is performed, rather than massive AMPure® bead-based clean-ups. Additional library quantification analysis such as TapeStation® analysis or gel electrophoresis are not necessary. With these features, the workflow dramatically reduces the hands-on time of library preparation to only 30 minutes.

Highlights for Quick-16S Plus NGS Library Prep Kit (V3-V4, UDI):

• The most streamlined NGS kit with only 30 minutes of hands-on time for 96 samples.
• 100% automation ready with only a single PCR step and without the need for normalization.
• Real-time PCR enables absolute microbial copy number quantification.

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