Minicircle Cloning Services

Do you want sustained transgene expression without the risks of introducing exogeneous DNA, such as for developing animal models or pioneering new gene therapy approaches?

SBI's minicircle technology is a great option. If you don’t have time to spend constructing and producing minicircles or the in-house expertise you can make use of SBI Custom Minicircle Services.

Why choose SBI’s CRISPR/Cas9 genome engineering services?

• Experienced scientific team that routinely produces minicircles for many customers including large pharma partners
• They clone your cDNA, miRNA, or shRNA into one of their highly-regarded minicircle parental vectors and produce large quantities of minicircles—or only parts of the cloning and production process. They can even customize the parental vector for you.
• Whether you need 250 µg or 10 mg of minicircles, it can be delivered taking as little as four weeks.
• Access Minicircle Technology for research use with no licensing restrictions when ordering ready-to-use minicircle DNA through the production service
• Leverage the minicircle platform for durable gene expression in your most demanding applications

Ordering Minicircle Services is easy

Step 1. Contact the services team for a quote by emailing support@sanbio.nl 
Step 2. Send us your producer plasmid, or have us make the vector for you
Step 3: Receive ready-to-transfect minicircle plasmids as little as four weeks

Produced as small circular DNA fragments after excision from a parental plasmid, the non-viral, episomal minicircle expression cassette is free of any bacterial plasmid DNA sequences that can lead to silencing, and comes with a variety of promoter and reporter combinations. Their small size facilitates more efficient transfection than what’s possible with standard-sized plasmids, and, while minicircles do not replicate with the host cell, expression lasts for 14 days or longer in dividing cells, and can continue for months in non-dividing cells.