NF-kB (p65) NLS Polyclonal Antibody

NF-?B p65 is a ubiquitously expressed transcription factor that is a subunit of the NF-?B complex and is encoded by the RELA gene in humans.{53059} It is composed of an N-terminal Rel homology domain, which contains the nuclear localization signal (NLS), and mediates dimerization, nuclear localization, and DNA and protein interactions, and two C-terminal transactivation domains that are subject to a variety of post-translational modifications and regulate the transcriptional activity of p65.{53059,53060} NF-?B p65 regulates the expression of a large number of genes in response to inflammatory and environmental cues that play critical roles in innate and adaptive immunity and cellular differentiation.{53060} Silencing of Rela induces tumor cell apoptosis in a murine Lewis lung carcinoma model, and RELA silencing in THP-1 monocytes decreases secreted levels of IL-1? and TNF-? induced by LPS.{53061,53062} Genome-wide deletion of Rela in mice is embryonic lethal.{5321} NF-?B p65 is overexpressed in the inflamed joints of patients with rheumatoid arthritis, and naïve CD4 T cells isolated from the whole blood of patients with multiple sclerosis have increased phosphorylation of NF-?B p65.{53065,53066} Cayman's NF-?B (p65) NLS Polyclonal Antibody can be used for immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), IHC-paraffin (IHC-P), and Western blot (WB) applications. The antibody recognizes the NLS region of NF-?B to detect NF-?B at 65 kDa from human samples. It also recognizes the NLS region of NF-?B from chimpanzee, cow, gorilla, horse, monkey, and mouse samples.