CD83, Human, mAb ELBE-1

Monoclonal antibody ELBE-1 recognizes human CD83. CD83 is a cell surface marker predominantly expressed on mature DCs. Expression has been found on circulating and tissue DCs. Additionally CD83 is found on activated B and T lymphocytes. Human CD83 is composed of 205 amino acids but has, due to extensive glycosylation, a molecular weight of ca 45 kDa. CD83 is expressed as a conserved a noncovalently associated single chain molecule membrane protein. CD83 is considered a marker for fully matured dendritic cells in the peripheral circulation. An essential role has not been found. However, its function primarily lies in the regulation of T- and B-cell maturation and the regulation of their peripheral responses. CD83 is supposed to function as a co-stimulator for T-cell activation similar to CD80 or CD86. Enhanced expression on DCs correlates with their T cell activating capacity. Besides the membrane version of the protein also a soluble form occurs (sCD83). sCD83 levels are raised in sera from patients of chronic lymphocytic leukemia and other haematological malignant diseases and in the synovial fluid of rheumatoid arthritis. While the membrane bound form has immune stimulatory capacity, the soluble form has the opposite effect and is considered to have immunosuppressive potential. Due to its inhibitory functions, sCD83 has potential for treatment of eg. autoimmune diseases and transplantation. In contradiction to most clones, antibody ELBE-1 is besides flow cytometry applicable in western blotting and ELISA (detecting of sCD83). ELBE-1 recognizes a linear epitope and doesn€™t influence T cell activation.