Goat anti Rat IgG1 IgG2a IgG2b IgG2c (Fc specific), conjugated with Horseradish peroxidase

The reactivity of the antiserum is directed to the Fc subunit of the IgG molecule. The antiserum contains antibodies to subclass-specific determinants as well as to determinants shared by two or more subclasses of IgG. In immunoelectrophoresis and double radial immunodiffusion, using various antiserum concentrations against normal rat plasma, serum, and immunoglobulin fractions, no reaction is obtained with IgA, IgM and gG/Fab fragments or any other serum protein. In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgG at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgG antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of rat origin known to be of the IgG isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgG in rat serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.