Goat anti Rabbit IgG IgA IgM (heavy and light chains), conjugated with TRITC

The reactivity of the antiserum is directed to the Fc and Fab subunits of the major immunoglobulins isotypes It does not react with any non-immunoglobulin in rabbit serum, as tested by immunoelectrophoresis and double radial immunodiffusion. To identify and measure immunoglobulins, antigen or antibody, at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates. If two Nordic immunoconjugates with different labels but originating from the same host animal species are used for direct two-color staining, they can safely be mixed and used in a single step, without the danger of interaction between the two reagents. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.