Goat anti Human antithrombin III

The reactivity of the antiserum is restricted to antithrombin III. In immunoelectrophoresis and radial immunodiffusion (Ouchterlony), using various antiserum concentrations against fresh normal human plasma a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified antithrombin III. No reaction is obtained with any other plasma protein including the main proteinase inhibitors known to have structural homology with antithrombin III. Protein complexes containing antithrombin III may also react. The anodic mobility enables quantitative determination of antithrombin III using the standard EID technique (Laurell). Immunoelectrophoresis and radial immunodiffusion are used to identify antithrombin III and enzyme inhibitor complexes. Double radial immunodiffusion (Mancini) is also used for quantitative assays. If applied in more sensitive test procedures, in Immunohistochemistry, or solid phase immunoassays, appropriate specificity controls should always be included. Plasma samples and all assay components must contain EDTA to stabilize the proteins.