BUF054A is a high performance TMB (3,3´, 5, 5´- tetramethylbenzidine) solution, recommended for use in ELISA detection systems as a substrate for horseradish peroxidase (HRP).
BUF054A contains TMB, substrate buffer and hydrogen peroxide in a safe, ready to use solution. TMB SIGNAL+ has been optimised to enable increased sensitivity whilst maintaining increased stability. BUF054A provides minimal background and rapid development.
BUF054A produces a deep blue colour during the enzymatic degradation of H2O2 by horseradish peroxidase. For kinetic assays the absorbance is read at 655nm (blue). However, for endpoint assays the
reaction may be stopped with 0.2M sulphuric acid, resulting in a yellow colour read at 450nm.
Productname
TMB Signal
BUF054A
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Productname
TMB Signal
BUF054A
By filling out this form, you request a sample. You will receive an order confirmation within one working day. The order cannot be modified after receipt of the order confirmation.
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