HISPEC assay diluent works to reduce cross reactivity, non-specific binding and matrix effects in immunoassays such as ELISA, EIA, Western blotting, immuno-PCR, protein arrays, multianalyte immunoassays and immunohistochemistry. The effect of HISPEC assay diluent is dependent on assay system and the antibodies used.
HISPEC assay diluent is used instead of a sample buffer or antibody dilution buffer within the immunoassay protocol.
HISPEC assay diluent is unsuitable for blocking of surfaces. For blocking of surfaces we recommend one of the blocking solutions within the Bio-Rad range – see “Useful reagents” below.
Examples of use:
ELISA: use as a dilution buffer for the specimen and for the detection antibodies
Western blotting: use as dilution buffer for primary and secondary antibodies
Immunohistochemistry: use as dilution buffer for primary and secondary antibodies
Protein arrays: use as dilution buffer for specimen and for the detection antibodies
Productname
Hispec Assay Diluent
BUF049A
By filling out this form, you are placing an order by e-mail. You will receive an order confirmation within one working day. The order cannot be modified after receipt of the order confirmation.
Productname
Hispec Assay Diluent
BUF049A
By filling out this form, you request a sample. You will receive an order confirmation within one working day. The order cannot be modified after receipt of the order confirmation.
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