Elisa IgM reducing Assay Diluent

BUF038B is an ELISA assay diluent designed to reduce background noise and assist in matrix equalization in problematic serum and plasma samples, especially those affected by high levels of “sticky” IgM which can non-specifically bind the HRP conjugate. Specially formulated for use in sandwich ELISA, it contains mammalian protein additives based on normal goat serum to reduce non-specific interactions between sample proteins and the plate surface. The difference in complexity, between serum or plasma samples and diluted standard curve proteins, is a potential cause of target analyte under-recovery. BUF038B equalizes the matrix, reduces IgM-mediated bridging interference, inhibits complement and thrombin activity and contains an antimicrobial agent.