anti-DDDDK tag antibody (FG4R)

Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein. The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function. The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins.Cleavage catalyzed by Cu2+ ions of the C-terminal xxxDDDDK from a fusion protein has been reported (Humphreys, D.P., et al., 1999). A sequence motif with five out of eight amino acid residues identical to the xxxDDDDK is found in both rat and mouse Mg2+ dependent protein b-phosphatase, as well as in the human and bovine enzyme.