H 206 allows demonstration of C3 deposits in tissue, on cells, on microorganisms and in immune complexes. It does not react with C3a or C3d and does not inhibit hemolytic function of C3. The epitope was located on the C-termius of the alpha-chain (39.5 kD fragment of C3c).
Disorders specifically detected: Detection of C3b deposits in tissue, on cells, on microorganisms and in immune complexes. It should be used with positive control anti C3b-ß, (H11; Cat. No. 61020) and negative control anti-C3a (H13; Cat. No. 61018). Activated, deposited C3b bears C3b-a and C3b-ß, but no longer C3a. Ubiquitous or nonspecifically absorbed C3 is still C3a positive with H 13.
Polypeptide reacting: C3b alpha-chain.