CD45 glycoproteins have various molecular weights on various cell types: B-cell 240 kDa, thymocytes 180 kDa, T-cells multiple bands. Reduced in PAGE gels: 180 and 240 kDa. Relevant epitopes are termed CD45RA (exon 4), CD45RB (exon5), CD45RC (exon 6) and CD45R or CD45R0 (exons 4-6 spliced out). Bra11 reacts with CD45RB, which is expressed on mature B-lymphocytes and the majority of lymphomas and leukemia's of B-cell origin. CD45RB is also expressed on cells of myeloid lineage.
Positive control: Ramos, U-698, or GA-10 cells. Tonsil.