This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Human IL-18 antibody was pre-coated into 96-well plates. Biotin conjugated anti-human IL-18 detection antibody was used. Standards, test samples and biotin conjugated detection antibody were added to the wells subsequently. Wash buffer was used to wash any non-specific binding. HRP conjugated Streptavidin was used as secondary antibody. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human IL-18 amount of samples captured in the plate. Optical Density (O.D) can be read at absorbance 450 nm in a microplate reader. Concentration of Human IL-18 can be calculated using the standard curve.
Productname
Human IL-18 (Interleukin 18) Pre-Coated ELISA Kit
90-2008
By filling out this form, you are placing an order by e-mail. You will receive an order confirmation within one working day. The order cannot be modified after receipt of the order confirmation.
Productname
Human IL-18 (Interleukin 18) Pre-Coated ELISA Kit
90-2008
By filling out this form, you request a sample. You will receive an order confirmation within one working day. The order cannot be modified after receipt of the order confirmation.
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