anti-Butyrophilin, mouse monoclonal, clone 102.43

The mab reacts specifically with butyrophilin, a unique 67 kD polypeptide present in lactating mammary gland epithelium and in the milk fat globule membrane (MFGM). No cross-reaction with other MFGM proteins. The epitope has been localized on the N-terminal extracellular part of the protein, close to the transmembrane region. Butyrophilins have recently been described as a new family of immunoregulators, similar to the co-stimulatory and co-inhibitory family of B7 molecules (Arnett & Viney, 2014). Polypeptide reacting:Butyrophilin, 67 kD polypeptide; present in the apical membrane of lactating mammary epithelium and in MFGM. Reactivity on cultured cell lines: Transfected cell line BMGE with butyrophilin cDNA. Positive control: Milk fat globule membrane (MFGM). Arnett HA & Viney JL: Immune modulation by butyrophilins. Nature Review Immunology 14, 559-569 (2014)