anti-OATP2/OATP8, mouse, monoclonal, clone mMDQ

In Western blot analyses of basolateral membrane fractions of human liver the monoclonal antibody mMDQ reacts with 90 kD and 120 kD proteins (after SDS-PAGE) representing OATP2 (also described as OATP-C) and OATP8, respectively. According to a recent new nomenclature these hepatocyte-specific proteins are identical to OATP1B1 and OATP1B3, respectively (Hagenbuch et al., 2003). Using immunofluorescence microscopy mMDQ stains the sinusoidal (basolateral) membranes in frozen sections of human liver, hepatocellular carcinoma (Cui et al., 2003). Tested reactivities on cultured sell lines: Transfected HEK293 cells; negative with HepG2 and PLC Polypeptide reacting: OATP2 and OATP8, human organic anion transporters (90 kD, 120 kD, after SDS-PAGE) Hagenbuch, B. & Meier, P. J. The superfamily of organic anion transporting polypeptides. Biochim. Biophys. Acta 1609, 1–18 (2003).Cui, Y. et al. Detection of the human organic anion transporters SLC21A6 (OATP2) and SLC21A8 (OATP8) in liver and hepatocellular carcinoma. Lab. Invest. 83, 527–38 (2003).