13,14-dihydro-15-keto Prostaglandin F2a ELISA Kit

Prostaglandin F2? (PGF2?) is one of the five primary prostaglandins derived enzymatically directly from the endoperoxide PGH2. PGF2? was initially discovered in seminal fluid, and to date the majority of the functional roles ascribed to it relate to fertility, pregnancy, and parturition. PGF2? is a potent luteolytic agent and is used to induce ovulation in domestic livestock. It is also a potent uterine stimulant, and is part of the cascade of myometrial stimulants which induce and sustain labor. PGF2? is rapidly metabolized to 13,14-dihydro-15-keto PGF2? in vivo, by the enzymes 15-hydroxy prostaglandin dehydrogenase and ?13-reductucase.{310,1157,5442,387} Measurement of 13,14-dihydro-15-keto PGF2? in plasma can be used as a marker of the in vivo production of PGF2?.{878,1463} The Cayman Chemical 13,14-dihydro-15-keto PGF2? Enzyme Immunoassay is a competitive assay that provides measurements of 13,14-dihydro-15-keto PGF2? within the range of 2.3-5,000 pg/ml, typically with a detection limit (80% B/B0) of approximately 15 pg/ml. Although the tracer and antiserum for this assay have been sold separately for many years, the reagents are now combined with an accurate standard and packaged together in a convenient assay kit. The assay has been validated for the measurement of 13,14-dihydro-15-keto PGF2? in plasma, a common matrix for measurement of this PGF2? metabolite.{878}