Integrin a5B1 Chimeric Monoclonal Antibody

Integrin ?5?1 is a cell adhesion receptor that is involved in the attachment of cells to the extracellular matrix (ECM) and cell-cell interactions.{59572} It is composed of a heterodimer of two integrin subunits, integrin ?5, also known as CD49E, that binds to the RGD sequence and central cell-binding domain of fibronectin, and integrin ?1, also known as CD29, that interacts with the cytoskeleton and intracellular signaling molecules.{66151,59653} Integrin ?5?1 localizes to the cell surface and mediates outside-in and inside-out signaling cascades that regulate angiogenesis and cell differentiation, as well as migration, development, and hematopoiesis.{66152,59572} An anti-integrin ?5?1 monoclonal antibody inhibits tube formation and proliferation of human umbilical vein endothelial cells (HUVECs) in vitro and prevents laser-induced choroidal neovascularization in a cynomolgus monkey model of macular degeneration.{66153} Integrin ?5?1 levels are increased in tumoral neovasculature.{66154} Cayman's Integrin ?5?1 Chimeric Monoclonal Antibody was produced recombinantly from the original M200 antibody sequence and can be used for ELISA, flow cytometry (FC), immunofluorescence (IF), and immunohistochemistry (IHC) applications. The M200 antibody was generated by fusing human IgG4? constant domains to the antigen-binding domain of a mouse anti-integrin ?5?1 monoclonal antibody.{66153}