Detyrosinated a-Tubulin Rabbit Monoclonal Antibody

Detyrosinated ?-tubulin is a form of the cytoskeletal protein ?-tubulin that has been post-translationally modified by the tubulin carboxypeptidases vasohibin 1 (VASH-1) and VASH-2, which remove the C-terminal tyrosine from ?-tubulin.{61815} Detyrosination of ?-tubulin occurs in the cytosol and exposes two consecutive glutamate residues that can be further processed to form ?2- or ?3-tubulin. Detyrosinated ?-tubulin monomers can be re-tyrosinated by tubulin tyrosine ligase and be incorporated into microtubules again. ?-Tubulin detyrosination is associated with, but not sufficient for, increased stability of the microtubule and is involved in a variety of biological processes, including neuronal development, ?-oxidation, mitosis, and cardiomyocyte contraction.{61816,61817,61815,61818} Increased levels of detyrosination are associated with tumor development in vitro, have been found in tumor tissue from patients with breast cancer, and are positively correlated with breast cancer aggressiveness.{61819,61820} Cayman’s Detyrosinated ?-Tubulin Rabbit Monoclonal Antibody can be used for immunocytochemistry (ICC), immunofluorescence (IF), and Western blot (WB) applications.