Akt1 PH Domain Rabbit Monoclonal Antibody

Akt1, also known as protein kinase B? (PKB?), is a serine/threonine kinase belonging to the AGC kinase family and one of three Akt isoforms in mammals.{57326,59549} Akt kinases function downstream of activated tyrosine kinases and PI3K to regulate a variety of cellular processes, including cell size, growth, proliferation, and survival, as well as genome stability, glucose metabolism, and neovascularization.{59549} Akt1 is composed of an N-terminal pleckstrin homology (PH) domain, which binds to phosphatidylinositol-(3,4,5)-triphosphate (PIP3) and phosphatidylinositol-(3,4)-diphosphate (PIP2), a kinase domain, and a C-terminal regulatory hydrophobic motif.{59545} The Akt PH domain is required for membrane localization and activation of Akt.{59550} It binds PIP3 and PIP2 generated by PI3K, which is activated by a variety of growth factors, recruiting Akt to the plasma membrane where it is phosphorylated at threonine 308 and serine 473, resulting in its activation.{59549} A glutamic acid-to-lysine substitution at glutamic acid 17 (E17K) in the Akt1 PH domain leads to constitutive Akt1 membrane localization and activation and has been found in tumors isolated from patients with breast, colorectal, or ovarian cancer.{15097} Cayman's Akt1 PH Domain Rabbit Monoclonal Antibody can be used for immunohistochemistry (IHC) and Western blot (WB) applications.