B-RAF Rabbit Monoclonal Antibody

Rapidly accelerated fibrosarcoma (Raf) kinase is a serine/threonine kinase and component of the MAPK/ERK signaling pathway.{54387,54388} Upon activation by upstream RAS signaling, Raf dimerizes and phosphorylates MEK1 leading to activation of transcription factors for cell growth, proliferation, and survival.{54388} Raf exists as three isoforms, A-RAF, B-RAF, and C-RAF, that all consist of three conserved regions (CRs) with domain-specific functions.{54387} CR1 contains a cysteine-rich domain and a RAS-binding domain, CR2 is essential for negative regulation through inhibition of phosphorylation sites, and CR3 is the kinase domain. B-RAF is the most prominent isoform, expressed in most tissues, and localized to the cytosol. Activating mutations in B-RAF have been found in various cancers, including melanoma and non-small cell lung cancer (NSCLC), as well as in patients with Langerhans cell histiocytosis.{54387,54388,54570} Cayman’s B-RAF Rabbit Monoclonal Antibody can be used for immunohistochemistry (IHC) and Western blot (WB) applications.