Smac (C-Term) Rabbit Monoclonal Antibody

Smac is a mitochondrial protein involved in the initiation of apoptosis.{8246} It is a ubiquitously expressed ?-helical protein that forms homodimers.{8246,59207} It is localized to the mitochondria but is released following apoptotic stimuli into the cytoplasm, where it binds to inhibitor of apoptosis proteins (IAPs), which are overexpressed in a variety of cancer cells.{8246,59208} Smac binding to IAPs blocks their inhibitory effect on caspase-3, -7, and -9 and leads to the activation of apoptosis.{59209,59208} Knockout of DIABLO, the gene encoding Smac, in colon cancer cells in vitro induces resistance to apoptosis induced by NSAIDs and TRAIL, but not cisplatin (Item No. 13119), mitomycin C (Item No. 11435), or camptothecin (Item No. 11694).{59210} DIABLO knockout in mice does not induce changes in apoptosis or tumorigenesis, indicating a redundancy of its function.{59208} Overexpression of DIABLO in vitro does not affect basal levels of apoptosis but increases the sensitivity of cells to other apoptotic stimuli.{59211} Cayman’s Smac Rabbit Monoclonal Antibody can be used for immunohistochemistry (IHC) and Western blot (WB) applications.