Paxillina/B/y (N-Term) Rabbit Monoclonal Antibody

Paxillin is a focal adhesion adapter protein that facilitates the assembly of multiprotein complexes that mediate signal transduction between the extracellular matrix and actin cytoskeleton.{59175,59176,59177} It contains four LIM domains in the C-terminal region that target paxillin to focal adhesions and an N-terminal region with five LD motifs that serve as docking sites for a variety of proteins, including vinculin, focal adhesion kinase (FAK), and Src, that facilitate intracellular signal transduction.{59177} Alternative splicing of PXN generates three isoforms, ?, ?, and ?, that contain a variable N-terminus, as well as a fourth isoform, ?, that lacks the N-terminal region.{59177} Paxillin is expressed in most tissues and predominately localizes to focal adhesions on the cell membrane.{59176,59177} Upon cytokine or growth factor stimulation or cell adhesion, paxillin is phosphorylated by a variety of kinases, including FAK and ERK, providing a scaffold for signaling proteins that are involved in the formation and regulation of focal adhesions, which mediate cell migration.{59177} It is also expressed in the cytoplasm and nucleus where it regulates gene transcription. Tumor paxillin levels are increased in patients with glioblastoma multiforme (GBM) and associated with poor survival. Cayman’s Paxillin?/?/? (N-Term) Rabbit Monoclonal Antibody can be used for immunocytochemistry (ICC) and Western blot (WB) applications.