Akt1 (C-Term) Rabbit Monoclonal Antibody

Akt1, also known as protein kinase B? (PKB?), is a serine/threonine kinase belonging to the AGC kinase family and one of three Akt isoforms in mammals.{57326,59549} Akt kinases function downstream of activated tyrosine kinases and PI3K to regulate a variety of cellular processes, including cell size, growth, proliferation, and survival, as well as genome stability, glucose metabolism, and neovascularization.{59549} Akt1, like Akt2 and Akt3, is composed of an N-terminal pleckstrin homology (PH) domain, which binds to phosphatidylinositol-(3,4,5)-triphosphate (PIP3) and phosphatidylinositol-(3,4)-diphosphate (PIP2), a kinase domain, and a C-terminal regulatory hydrophobic motif. It is ubiquitously expressed and is the primary isoform in endothelial cells.{15560} Akt1 is activated via recruitment to the plasma membrane, which is mediated by the interaction of the Akt1 PH domain with PI3K-generated PIP3 and PIP2, and subsequent phosphorylation at threonine 308 and serine 473.{59549} Increased Akt1 kinase activity has been found in tumor tissue isolated from patients with prostate, breast, and ovarian cancers.{59550} Cayman’s Akt1 (C-Term) Rabbit Monoclonal Antibody can be used for immunohistochemistry (IHC) and Western blot (WB) applications. The antibody recognizes Akt1 at approximately 56 kDa from human samples.