p38a MAPK Rabbit Monoclonal Antibody (Clone RM245)

p38 MAPK is a serine/threonine protein kinase and member of the MAPK family with roles in the regulation of immune responses and embryonic development, as well as cell differentiation, metabolism, and survival.{60066,60067} It exists as 4 isoforms, p38?, -?, -?, and -?, encoded by MAPK14, MAPK11, MAPK12, and MAPK13, respectively, in humans. p38? MAPK is ubiquitously expressed, with the highest levels of expression in heart, skeletal muscle, and brain.{60066,60068} It is activated via dual phosphorylation of threonine 180 and tyrosine 182 by the MAP2K kinases MKK3 and MKK6 in response to LPS or the production of inflammatory cytokines.{60066,60067} Downstream signaling targets of p38? MAPK include protein kinases, transcription factors, and transcriptional regulators, among others.{60067} Knockdown of Mapk14 is embryonic lethal, while macrophage-specific deletion of Mapk14 inhibits inflammatory cytokine production and is protective against cecal ligation and puncture-induced sepsis in mice.{60067,60068} Mapk14 knockdown also increases lysosomal degradation of ?-secretase 1 (BACE1) and decreases amyloid-? (A?) production in the APP/PS1 double transgenic mouse model of Alzheimer's disease.{60069} Cayman's p38? MAPK Monoclonal Antibody (Clone RM245) can be used for immunohistochemistry (IHC) and Western blot (WB) applications. The antibody recognizes p38? MAPK at approximately 38 kDa from human samples.