HIF-1a (human) Monoclonal Antibody

Hypoxia-Inducible Factor-1? (HIF-1?) is a transcription factor subunit that belongs to the basic helix-loop-helix PER-ARNT-SIM (bHLH-PAS) protein family.{11044,46550} It contains bHLH and PAS domains that mediate DNA binding and heterodimerization with the HIF-1? subunit, an oxygen-dependent degradation (ODD) domain that is hydroxylated by prolyl hydroxylase in the presence of oxygen to target HIF-1? for proteasomal degradation, and N- and C-terminal transactivation domains responsible for regulating the expression of HIF-1 target genes.{46550,46551} Under hypoxic conditions, HIF-1? is stabilized, accumulates in the cytoplasm, and is translocated to the nucleus where it forms a heterodimer with HIF-1? and induces the expression of genes involved in maintaining cellular oxygen homeostasis.{11044,46550,10948,12428} It is also involved in angiogenesis, glucose utilization, and pH regulation under hypoxic conditions, including in the tumor microenvironment.{17023} HIF-1? is overexpressed in a variety of cancer cell lines where it promotes survival of cancer cells and increases invasiveness under hypoxic conditions and, in vivo, overexpression is associated with aggressiveness and progression of various cancers and poor disease-free survival.{17023,9833,46552,46553} Homozygous knockout of HIF-1? is embryonic lethal due to disruptions in vascular development but conditional knockout models have demonstrated a role for HIF-1? in inflammation, immunity, and osteogenesis.{17023} Cayman's HIF-1? (human) Monoclonal Antibody can be used for immunohistochemistry (IHC) and Western blot (WB) applications. The antibody recognizes HIF-1? at approximately 120 kDa from human samples.