Histone H3T11Ph Monoclonal Antibody

Histone H3 is a nuclear protein and a component of the nucleosome core, a basic unit of chromatin, that is essential for organizing genomic DNA in eukaryotic nuclei.{57056} It is a globular protein that contains an unstructured N-terminal tail that extends outside of the nucleosome core and is subject to various post-translational modifications (PTMs), including methylation, phosphorylation, acetylation, and citrullination.{57056,53978} Phosphorylation of histone H3 at threonine 11 (H3T11Ph) occurs during mitosis in mammalian cells and localizes to the centromeric regions of chromosomes during metaphase.{54373} H3T11 can be phosphorylated by multiple kinases such as PKC-related kinase (PRK1), which mediates androgen-induced phosphorylation of H3T11 at the androgen receptor target genes PSA and KLK2 in LNCaP cells, and checkpoint kinase 1 (Chk1), which phosphorylates H3T11 at the promoter regions of the genes encoding cyclin B1 and Cdk1 in mice. {54374,54375} High levels of H3T11Ph in tumor tissue isolated from patients with prostate cancer correlate with high Gleason scores, which are associated with aggressive tumors.{54374} Cayman’s Histone H3T11Ph Monoclonal Antibody can be used for ELISA, multiplex-based assays, and Western blot (WB) applications.