Histone H3K79Me2 Monoclonal Antibody

Histone H3 is a nuclear protein and a component of the nucleosome core, a basic unit of chromatin, that is essential for organizing genomic DNA in eukaryotic nuclei.{57056} It is a globular protein that contains an unstructured N-terminal tail that extends outside of the nucleosome core and is subject to various post-translational modifications (PTMs), including methylation, phosphorylation, acetylation, and citrullination.{57056,53978} Dimethylation of histone H3 at lysine 79 (H3K79Me2) is a cell cycle-dependent modification associated with gene repression that is low during G1 and early S phases, increases during late S phase, and is maintained during G2/M.{57208} Levels of H3K79Me2 are increased during mitosis in a histone H2B monoubiquitination-dependent manner, and loss of H3K79Me2 is associated with increased chromosome numbers and mitotic defects in HCT116 cells.{57209} H3K79Me2 is completely absent in postmortem brain tissue isolated from fetuses with spina bifida.{57207} Cayman’s Histone H3K79Me2 Monoclonal Antibody can be used for chromatin immunoprecipitation (ChIP), ELISA, immunohistochemistry (IHC), multiplex-based assay, and Western blot (WB) applications.