IgG2c (mouse) Rabbit Monoclonal Antibody

Immunoglobulin G (IgG) is a member of the immunoglobulin superfamily of glycoproteins that plays a central role in the adaptive immune response.{28520} It is produced by B cells and later secreted by plasma cells and is the most abundant circulating antibody in human and mouse serum.{28520,55170,55174} IgG consists of two heavy chains of approximately 50 kDa each and two light chains of approximately 25 kDa each.{28520} The heavy chains are linked together by disulfide bonds to form an Fc region and also combine with the light chains to form the Fab region, which mediate receptor and antigen binding, respectively.{55171} IgG is produced following IgM class-switching in response to infection and is involved in numerous humoral host defense responses, including antibody-dependent cell-mediated cytotoxicity (ADCC), toxin neutralization, and pathogen opsonization.{55170} IgG exists as four isotypes in mice: IgG1, IgG2b, IgG3, and, in a strain-specific manner, IgG2a or IgG2c.{55172,53858} IgG2c is encoded by Ighg2c and is expressed in certain inbred mouse strains, such as C57BL/6, C57BL/10, SJL, and NOD mice.{53858} Class switching to the IgG2c isotype occurs via IFN-? stimulation during the early immune response.{56168} IgG2c binds to the high affinity Ig? Fc receptor I (Fc?RI) on dendritic cells and the low affinity Fc?RIII and Fc?RIV, which are expressed on a variety of immune cells, and is involved in complement activation.{56169,56170} Cayman's IgG2c (mouse) Rabbit Monoclonal Antibody (Clone RM223) can be used for ELISA and Western blot (WB; non-reducing conditions) applications. The antibody recognizes the Fc region of IgG2c from mouse samples.