Cardiac Troponin I Polyclonal Antibody

Cardiac troponin I (cTnI) is a subunit of the troponin complex in cardiac muscle thin filaments, which plays a role in muscle activity.{55210} It is encoded by TNNI3 in humans and is expressed exclusively in the heart. cTnI is a multidomain protein composed of an N-terminal domain, IT-arm, inhibitory domain, regulatory domain, and C-terminal mobile domain. The N-terminal domain is exclusive to cTnI and is essential to the interaction of cTnI with troponin C and in the regulation of calcium-dependent muscle contraction. cTnI binds to actin to hold the troponin complex in place in thin myofilaments. Phosphorylation of cTnI at serines 22 and 23 in the N-terminus decreases sarcomere calcium sensitivity, promotes calcium dissociation from troponin C, and enhances diastolic relaxation.{55219,55221} Cardiac systolic dysfunction in human and animal models of heart failure is linked to PKC phosphorylation of cTnI at serines 23, 24, 43, and 45, as well as threonine 144.{55221} Cayman’s Cardiac Troponin I Polyclonal Antibody can be used for Western blot (WB) applications. The antibody recognizes cTnI at approximately 25 kDa from human, mouse, and rat samples.