SNAP-25 (Phospho-Ser187) Polyclonal Antibody

Synaptosomal-associated protein 25 (SNAP-25) is a member of the SNARE complex, which also includes syntaxin and VAMP, that is responsible for fusing synaptic vesicles with the presynaptic plasma membrane to facilitate neurotransmitter release.{55160} Two SNAP-25 isoforms, SNAP-25a and SNAP-25b, are generated through alternative splicing, with SNAP-25b expressed only during the postnatal period and as the predominant isoform in the brain.{55161} SNAP-25 contains two ? helices, as well as one large and several smaller intrinsically disordered domains.{55160} SNAP-25 is located primarily on the intracellular side of the presynaptic plasma membrane in neurons and interacts with a variety of proteins to orchestrate vesicle fusion in a calcium-triggered manner and to mediate spine development.{55160} It is also found in the pancreas, enteroendocrine cells, and the chromaffin cells of the adrenal medulla where it is involved in hormone secretion.{55161} SNAP-25 can be phosphorylated at serine 187 (Ser187) in a neuronal activity-dependent manner by PKC, a modification that increases the rate of synaptic vesicle recruitment and is essential for SNAP-25b inhibition of voltage-gated calcium channels (VGCCs) and incorporation of NMDA receptors into the postsynaptic membrane.{55154,55155,55156} Phosphorylation at Ser187 also facilitates neurotransmitter release and inhibits presynaptic short-term plasticity via regulation of synaptic vesicle dynamics.{55157} A point mutation at Ser187 (S187A) induces anxiety-like behavior in mice homozygous for the mutation and induces working memory deficits and an immature phenotype in hippocampal dental granule cells of adult mice heterozygous for the mutation.{55158,55159} Cayman’s SNAP-25 (Phospho-Ser187) Polyclonal Antibody can be used for Western blot (WB) applications. The antibody recognizes SNAP-25 (phospho-Ser187) at approximately 25 kDa from mouse and rat samples.