KCC2 (Phospho-Thr1007) Polyclonal Antibody

The neuronal-specific potassium chloride cotransporter 2 (KCC2) is a member of the SLC12 family of transporters and is encoded by SLC12A5 in humans.{55121} KCC2 is expressed in mature neurons throughout the CNS and localizes to the soma and dendrite plasma membrane where it mediates chloride ion efflux, maintaining the transmembrane chloride potential.{55122} It consists of 12 transmembrane helices, which contain an extracellular loop subject to N-glycosylation, as well as intracellular N- and C-terminal domains with sites that are subject to phosphorylation. Phosphorylation of KCC2 at threonine 1007 (Thr1007) is mediated by lysine-deficient protein kinase 1 (WNK1) and its effectors, STE20/SPS1-related proline-alanine-rich protein kinase (SPAK) and oxidative stress-responsive kinase 1 (OSR1), resulting in reduced KCC2 activity. Phospho-Thr1007 levels are decreased by the KCC2 activator N-ethylmaleimide (NEM) in HEK293 cells.{59146} Point mutation of Thr1007 (T1007E) in KCC2 mimics its phosphorylation and abolishes NEM-induced chloride ion efflux in HEK293 cells. Cayman's KCC2 (Phospho-Thr1007) Polyclonal Antibody can be used for Western blot (WB) applications. The antibody recognizes KCC2 (phospho-Thr1007) at approximately 135 kDa from mouse and rat samples.