Nestin Monoclonal Antibody

Nestin, also known as neuroepithelial stem cell protein, is a type VI intermediate filament protein.{55198} Nestin monomers are comprised of a short N-terminal head domain that is required for assembly of intermediate filaments, an ?-helical rod domain that mediates dimerization, and a long C-terminal tail.{55198} They form heterodimers and heterotetramers with other intermediate filament proteins, including vimentin and ?-internexin, for assembly into intermediate filaments.{55198,55199} Nestin is found in a variety of tissue types but is expressed primarily in nervous tissue during embryonic development in mammals and has been used as a marker of neural stem/progenitor cells (NSPCs).{55199,55200} Nestin synthesis decreases as nervous tissues differentiate, however, nestin-positive neural stem cells are found in the subventricular zone (SVZ) and hippocampal dentate gyrus in the adult mammalian brain. Nestin is also expressed in a variety of tumor types and cancer stem cells, as well as in cerebellar Purkinje cells from patients with Creutzfeldt-Jakob disease (CJD).{55200,55201} Cayman’s Nestin Monoclonal Antibody (Clone 4D11) can be used for immunocytochemistry (ICC), immunohistochemistry (IHC), and Western blot (WB) applications. The antibody recognizes a nestin doublet at approximately 220 to 240 kDa from human, mouse, and rat samples.