GABAA Receptor B3 Subunit Polyclonal Antibody

GABAA receptors are ligand-gated chloride channels that mediate the effects of the inhibitory neurotransmitter GABA in the CNS.{46949,46950} They are postsynaptic heteropentameric receptors that contain protein subunits from the following isoforms: ?1-6, ?1-4, ?1-3, ?, ?, ?, ?, and ?1-3, arranged around a central pore. Phasic inhibitory synaptic transmission is regulated by ?1?2?2 subunit-containing GABAA receptors, the major isoform found in the brain.{46950,46951} The ? subunit of GABAA receptors interfaces with an ? subunit to form the GABA binding site that initiates GABA-induced action potentials and forms the benzodiazepine binding site with the ? subunit. ?3 subunit-containing GABAA receptors are widely expressed in the cerebral cortex, cerebellum, olfactory bulb, and hippocampus.{55098} Phosphorylation of the ?3 subunit by PKA or PKC inhibits binding of the ?3 subunit with the clathrin adaptor protein AP2 and reduces GABAA receptor endocytosis.{55097} Mutations in GABRB3, which encodes the ?3 subunit isoform, have been found in patients with childhood absence epilepsy (CAE), infantile spasms (IS), and Lennox-Gastaut syndrome (LGS).{46950} Cayman's GABAA Receptor ?3 Subunit Polyclonal Antibody can be used for immunohistochemistry (IHC) and Western blot (WB) applications. The antibody recognizes the GABAA receptor ?3 subunit at approximately 53 kDa from rat and mouse samples.