GABAA receptors are ligand-gated chloride channels that mediate the effects of the inhibitory neurotransmitter GABA in the CNS.{46949,46950} They are postsynaptic heteropentameric receptors that contain protein subunits from the following isoforms: ?1-6, ?1-4, ?1-3, ?, ?, ?, ?, and ?1-3, arranged around a central pore. Phasic inhibitory synaptic transmission is regulated by ?1?2?2 subunit-containing GABAA receptors, the major isoform found in the brain.{46950,46951} The ? subunit of GABAA receptors interfaces with an ? subunit to form the GABA binding site that initiates GABA-induced action potentials and forms the benzodiazepine binding site with the ? subunit. Phosphorylation of the ?1 subunit by PKA or PKC inhibits binding of the ?1 subunit with the clathrin adaptor protein AP2 and reduces GABAA receptor endocytosis.{55097} Mice expressing mutations in Gabrb1, which encodes the ?1 subunit isoform, have increased GABAA receptor-mediated tonic inhibition in the nucleus accumbens and increased alcohol consumption compared to wild-type mice.{55212} SNPs in GABRB1 are associated with increased impulsivity and reward sensitivity in human adolescents.{55217} Cayman's GABAA Receptor ?1 Polyclonal Antibody can be used for Western blot (WB) applications. The antibody recognizes the GABAA receptor ?1 subunit at approximately 55 kDa from mouse and rat samples.