GABAA Receptor a6 Subunit Polyclonal Antibody

GABAA receptors are ligand-gated chloride channels that mediate the effects of the inhibitory neurotransmitter GABA in the CNS.{46949,46950} They are postsynaptic heteropentameric receptors that contains protein subunits from the following isoforms: ?1-6, ?1-4, ?1-3, ?, ?, ?, ?, and ?1-3, arranged around a central pore. Phasic inhibitory synaptic transmission is regulated by ?1?2?2 subunit-containing GABAA receptors, the major isoform found in the brain.{46950,46951} The ? subunit of GABAA receptors interfaces with a ? subunit to form the GABA binding site that initiates GABA-induced action potentials and forms the benzodiazepine binding site with the ? subunit. The GABAA receptor ?6 subunit is expressed in granule cells of the cerebellum and cochlear nucleus.{46947} Expression of GABRA6, which encodes the ?6 subunit isoform, is decreased in withdrawal seizure-prone, but not withdrawal seizure-resistant, mice after chronic ethanol administration. Point mutation of the arginine residue at position 100 (R100Q) of the GABAA receptor ?6 subunit increases sensitivity to diazepam and ethanol in rats and humans.{46948} Cayman’s GABAA Receptor ?6 Subunit Polyclonal Antibody can be used for Western blot (WB) applications. The antibody recognizes the GABAA receptor ?6 subunit at approximately 57 kDa from mouse and rat samples.