CaMKII (Phospho-Thr286) Polyclonal Antibody

Calcium/calmodulin-dependent protein kinase II (CaMKII) is a serine/threonine protein kinase and member of the calcium/calmodulin-activated protein kinase family.{53680,15705,15706} There are four isoforms of CaMKII, ?, ?, ?, and ?, encoded by CaMK2A, CaMK2B, CaMK2G, and CaMK2D in humans.{53680} CaMKII? and CaMKII? are predominantly expressed in neuronal tissue, while CaMKII? and CaMKII? are primarily found in cardiac tissue, however, the various isoforms of CaMKII can be found in nearly all cell types. Upon calcium binding, CaMKII undergoes a conformational switch that displaces the autoinhibitory domain inducing autophosphorylation of threonine 286 (Thr286) and autonomous activation of the kinase.{15712} Activation of CaMKII in dendritic spines in response to increased calcium concentration facilitates the induction of long term potentiation (LTP) and memory formation in rodents.{53681} Phospho-site mutant Camk2aT286A knock-in mice exhibit impaired LTP, as well as learning and memory deficits.{53681} Cayman’s CaMKII (Phospho-Thr286) Polyclonal Antibody can be used for immunohistochemistry (IHC) and Western blot (WB) applications. The antibody recognizes CaMKII? phospho-Thr286 and CaMKII? phospho-Thr286 at approximately 50 and 60 kDa, respectively, from human, mouse, rabbit, and rat samples.