Citrullinated Myelin Basic Protein Polyclonal Antibody

Citrullinated myelin basic protein (citMBP) is a citrullinated form of MBP, a protein that is integral to myelin stability in the CNS.{42873} MBP contains 19 arginine residues that can be citrullinated by peptidylarginine deiminases (PADs), decreasing the positive charge of MBP.{42873,42874} The loss of positive charge disrupts MBP-lipid interactions, leading to myelin destabilization and loss and increases its susceptibility for degradation. MBP is degraded by the myelin-associated protease cathepsin D, which leads to exposure and release of immunodominant epitopes.{42873,42875} The percentage of MBP that is citrullinated is increased in the postmortem brain from patients with multiple sclerosis (MS), with an even greater percentage citrullinated in Marburg disease, a more severe form of MS.{42873} citMBP isolated from patients with multiple sclerosis (MS) is degraded by cathepsin D at a higher rate than MBP isolated from healthy controls.{42873,42875} Cayman’s Citrullinated Myelin Basic Protein Polyclonal Antibody can be used for Western blot and ELISA applications. The antibody recognizes citMBP at 21 kDa from human samples, and was generated using a peptide immunogen containing citrullines at R122 and R130 (human myelin basic protein sequence, isoform 5).