COX-2 Monoclonal Antibody (Clone 12C10)

Cyclooxygenase 2 (COX-2) is a bifunctional enzyme that exhibits both COX and peroxidase activities and catalyzes the first step in the biosynthesis of prostaglandins, thromboxanes, and prostacyclins.{500,501} The COX component converts arachidonic acid (Item Nos. 90010 | 90010.1 | 10006607) to the hydroperoxy endoperoxide prostaglandin G2 (PGG2; Item No. 17010), and the peroxidase component reduces the endoperoxide to the corresponding alcohol PGH2 (Item No. 17020). COX2 expression is induced by a variety of stimuli, including phorbol esters, LPS, and cytokines and is responsible for the biosynthesis of PGs under acute inflammatory conditions.{54420,14766} Thus, COX-2 has been the focus of attention for nonsteroidal anti-inflammatory drug (NSAID) development. Cayman's COX-2 Monoclonal Antibody (Clone 12C10) can be used for ELISA, flow cytometry (FC), immunofluorescence (IF), and Western blot (WB) applications. The antibody recognizes a unique C-terminal region of COX-2 that is not present in COX-1, specifically detecting COX-2 at 72 kDa from human, mouse, and ovine samples.