Histone H1.4 (Citrullinated R53) Polyclonal Antibody

Citrullinated histone H1.4 is a citrullinated form of histone H1.4, a nuclear protein that interacts with linker DNA regions between nucleosomes to provide nucleosome core structure stabilization.{52742} Histone H1 subtypes are comprised of a short N-terminal domain, a central globular domain necessary for DNA interaction, and long C-terminal domain that determines subtype binding properties by its variability. Histone H1.4 is expressed in most somatic cell types and is essential for survival of T47D and MCF-7 breast cancer cells and MCF-10A breast epithelial cells.{52743,52744,52745} It is subject to citrullination by protein-arginine deiminase 4 (PAD4) at arginine 54, which corresponds to arginine 53 of the immunizing peptide for Cayman’s Histone H1.4 (Citrullinated R53) Polyclonal Antibody. Citrullination releases histone 1 from chromatin, leading to chromatin decondensation in mouse embryonic stem cells.{52746} Cayman’s Histone H1.4 (Citrullinated R53) Polyclonal Antibody can be used for Western blot applications. The antibody recognizes citrullinated histone H1, and not unmodified H1, at 35 kDa from human samples.