Prostaglandin E Synthase (cytosolic) Polyclonal Antibody

Prostaglandin E synthase (PGES) catalyzes the isomerization of PGH2 (Item No. 17020) to PGE2 (Item No. 14010). Cytosolic and microsomal PGES enzymes have been cloned and characterized. Cytosolic PGES (cPGES) is a glutathione-dependent enzyme with a predicted size of 18.6 kDa (23 kDa on SDS-PAGE). The enzyme is expressed in a wide variety of tissues and cells, the levels of which are unaffected by treatment with IL-1? and TNF-?.{8691} However, enzyme expression increases approximately 3-fold in rat brain following LPS treatment.{8691} Microsomal PGES (mPGES) is a 16 kDa protein expressed in a variety of tissues including prostate, testes, and small intestine, as well as in A549 and HeLa cells.{7229} In contrast to cPGES, mPGES protein expression is increased in A549 cells following treatment with IL-1?.{7229} The two enzymes show <10% homology at the amino acid level.