eIF4E (human recombinant; GST-tagged)

Eukaryotic translation initiation factor 4E (eIF4E) is a component of the eIF4 complex that specifically binds to the methy-7-guanosine cap structure at the 5’ end of eukaryotic mRNA to initiate translation.{23537,25363,41706} Nuclear eIF4E promotes export of mRNAs that contain the eIF4E sensitivity element (4E-SE) to the cytosol while cytosolic eIF4E, in a complex with eIF4A and eIF4G1/eIF4G3, mediates recruitment of mRNAs to the ribosome to initiate cap-dependent translation.{25357,41706} eIF14E activity is regulated by eukaryotic translation initiation factor 4E-binding proteins (4E-BPs), which bind eIF4E to prevent eIF4 complex assembly and subsequent translation.{41706} Overexpression of eIF4E is observed in approximately 30% of human malignancies, including breast, colon, and head and neck carcinomas, and acute myeloid leukemias, particularly M4 and M5 subtypes, as well as prostate cancers.{25358,25362,41706} Increased eIF4E expression leads to increased translation of oncogenic mRNAs, such as those encoding VEGF and cyclin D1, and contributes to cancer cell survival and proliferation.