Ro 3306

Cyclin-dependent kinase 1 (Cdk1) complexes with different cyclins, with the cyclin determining substrate specificity. Ro 3306 is a cell-permeable, reversible inhibitor of Cdk1, showing preference for Cdk1/cyclin B1 (Ki = 35 nM) over Cdk1/cyclin A (Ki = 110 nM), two CDK complexes which regulate cell cycling.{24139} It also inhibits Cdk2 (Ki = 340 nM), PKC? (Ki = 318 nM), and serum- and glucocorticoid-induce kinase (Ki = 497 nM) but not Cdk4.{24139} Through its effects on Cdk1, Ro 3306 reversibly arrests proliferating cells at the G2/M phase border, allowing cell synchronization without the use of microtubule poisons.{24140} Prolonged treatment of cells with Ro 3306 induces apoptosis and this can be augmented in acute myeloid leukemia cells by stimulating p53 activity with nutlin-3.{24141}