Anti-Dengue Virus (Clone: DENV-1C19)

Reactivity Species : Dengue Virus Expression Host : HEK-293 Endotoxin Level : ? 1.0 EU/mg as determined by the LAL method DENV-1C19 activity is directed against the bc loop of domain II of the E glycoprotein adjacent to the fusion loop (FL), is quaternary structure dependent, and cross-reactive against DENV-1, 2, 3, 4. Specificity: DENV-1C19 exhibited ultrahigh neutralization potency against strains corresponding to all four DENV serotypes. Fine epitope mapping studies revealed that DENV-1C19 recognizes a novel conserved site known as the bc loop (amino acids 73-79) adjacent to the fusion loop (FL) of DENV E protein in the DI/II hinge region. The bc loop residues 73, 78, and 79 have been identified as critical residues by loss-of-function binding screens. DENV-1C19 does not directly bind the FL and was unable to bind to wild-type E protein of West Nile Virus. DENV-1C19 binding was also not affected by alterations in DII-FL residues in yeast surface display or shotgun mutagenesis screenings. DENV-1C19 neutralizes DENV effectively and competes for binding against low-potency FL antibodies, which are believed to contribute to antibody-mediated disease1. When DENV-1C19 was tested in AG129 mice for protective efficacy, it reduced the level of viremia after sublethal virus challenge for DENV-1 and -2. DENV-1C19 was able to bind to four chimeric yellow fever-dengue vaccine viruses and detected all four serotypes equally in a dot plot.