LCAT Polyclonal Antibody

Lecithin:cholesterol acyltransferase (LCAT) has both phospholipase and acyltransferase activities. LCAT catalyzes the fatty acid transfer from the sn-2 position of phosphatidylcholine (lecithin) to cholesterol and to a lesser degree to other acceptor molecules.{14521,14519} This enzyme is critical to the process of reverse cholesterol transport or movement of cholesterol esters into high density lipoprotein (HDL) particles from cells.{13279} LCAT is abundant in blood-plasma, however it is present in other fluids and tissues such as ovary. This protein may be detected on immunoblot at 49.5 kDa as well as at 66 kDa when fully glycosylated.{14522,14520} Plasma samples should be diluted prior to electrophoresis and immunoblotting to avoid the band-broadening effect caused by comigration with endogenous albumin.