Prostaglandin Metabolite HPLC Mixture

This mixture contains the primary metabolites of prostaglandins (PGs) D2, E2, and F2?. Contents: 13,14-dihydro-15-keto PGD2, 13,14-dihydro-15-keto PGE2, 11?-PGF2?, 13,14-dihydro-15-keto PGF2?, and PGF2? (100 µg each). In primates, the lung is an active site of PG inactivation via 15-hydroxy PG dehydrogenase (15-hydroxy PGDH).{340} E and F-series PGs are good substrates for 15-hydroxy PGDH, which oxidizes the C-15 hydroxyl group to give the corresponding 15-keto compounds.{2309} This oxidation if followed rapidly by ?13-reductase removal of the 13,14-double bond, to give 13,14-dihydro-15-keto PG metabolites.{5442} While the primary PGs are often undetectable in plasma due to their rapid inactivation, the 13,14-dihydro-15-keto metabolites can be present at concentrations of several hundred pg/ml.{581} In addition, 9- and 11-keto PG-reductases have been reported, especially in the case of PGD2, which produce F-series PG metabolites with a ?-hydroxyl group on the ring.{2569} These metabolites span a wide polarity range and separate well on reversed phase HPLC.