p53 (Phospho-Ser392) Polyclonal Antibody

p53 is a transcription factor and tumor suppressor encoded by TP53 in humans with roles in cell cycle arrest, apoptosis, senescence, differentiation, and DNA repair.{59774,59775} It is composed of two N-terminal intrinsically disordered transactivation domains (TADs), a proline-rich domain (PRD), a DNA-binding domain (DBD), a tetramerization domain (TD), and an intrinsically disordered C-terminal domain.{59774} p53 is ubiquitously expressed, with protein levels increasing under conditions of extra- or intracellular stress, such as DNA damage, oncogene activation, oxidative stress, or hypoxia.{59776} It is activated and translocated to the nucleus in response to cellular stress via post-translational modifications, such as phosphorylation, methylation, and acetylation, where it binds p53 consensus DNA-binding elements and regulates transcription of its target genes in a cell-, tissue-, and stress signal type-specific manner.{59774,59776} p53 is phosphorylated at Ser392 by I?B kinase ? (IKK?) in glutamine-deficient cancer cells.{60852} Loss-of-function and/or gain-of-function mutations in TP53 occur in approximately 50% of human cancers.{59775} Cayman's p53 (Phospho-Ser392) Polyclonal Antibody can be used for Western blot (WB) applications. This antibody recognizes p53 at ~53 kDa from human and rat samples.